Enteric adenoviruses: detection, replication, and significance

Enteric adenoviruses: detection, replication, and significance.

Adenoviruses can be demonstrated readily in the stools of pediatric gastroenteritis patients by electron microscopy or counterimmunoelectrophoresis, but in 45% of these cases the virus will not grow in cell culture. Indirect immunofluorescence microscopy can be used to detect nongrowing strains of adenovirus; these strains have a unique single-cell fluorescence pattern. Hematoxylin and eosin st...

Incidence of enteric adenoviruses among children in Thailand and the significance of these viruses in gastroenteritis.

In countries with temperate climates, enteric adenoviruses have been shown to be a substantial cause of pediatric gastroenteritis. To determine the incidence of adenovirus infection in a tropical climate, stools were collected from children under age 7 during a 1-year period at an outpatient clinic in Bangkok, Thailand. Stools from 1,114 children with gastroenteritis and from 947 children witho...

Seroprevalence of enteric and nonenteric adenoviruses in Bangladesh.

Single serum samples obtained from infants between 0 and 24 months of age admitted to a diarrheal disease hospital in Bangladesh were tested for the presence of adenovirus-specific immunoglobulin G (IgG) and IgA antibodies by using enzyme immunoassay and neutralizing antibodies to adenovirus types 2, 40, and 41. IgG antibodies were more prevalent than IgA antibodies, and neutralizing activity t...

Isolation and propagation of enteric adenoviruses in HEp-2 cells.

Eighty-two stool samples from children with gastroenteritis in Canada, England, and Thailand which had been shown to contain adenovirus antigen (by a group-specific enzyme-linked immunosorbent assay) or adenovirus particles (by electron microscopy) or both, were tested for primary isolation of enteric adenoviruses in HEp-2 and Graham 293 cells. Graham 293 cells are known to support the replicat...

Replication-selective adenoviruses as oncolytic agents.